Basic transfer differential equation and transferring model of load transfer method were given at first, and the pile-soil loading state was divided into five stages according to the increasing pile-head loading process. Next, based on the overall equilibrium of pile and boundary conditions, analytical stiffness expressions of single pile relating to these transferring model parameters were derived in stages. And then, the field static load test result, Q-s curve, was also divided into five corresponding stages and subsection fitting was suggested to be made for each stage. Finally, each model parameter was determined one by one in stages according to the corresponding relations between the measured Q-s curve and the proposed analytical solutions. This pile-soil parameters determination method would enhance the application value of the measured Q-s curve in some extent, and it would provide a theoretical basis for further study on mechanical properties of pile-soil system.