In long-span cable-stay bridges, anchorage zone of cable-pylon is a key part to transfer cable force to pylon. Because of local concentrated force, irregular pylon section and complicated construction measures, a general mechanical analysis is unable to reflect actual stress distribution and working performance of anchorage zone. Based on Maling River Bridge in Guizhou province, China, clear finite element analysis and reliable full-scale model test for cable-pylon anchorage zone segment were carried out. Not only were design of model test and loading program introduced, but also test content and sensor arrangement were documented. In addition, details of finite element modeling were involved too, such as mesh generation, boundary condition and loading cases. Through comparison and analysis of stress increment and crack observation, the location where larger local tensile stress occurred was obtained. Corresponding anti-cracking load coefficient and safety coefficient of crack width were also presented. Parts of research findings have been used for the guidance of bridge construction.