This paper considers the demineralization of mine water using a lab-scale electrodialysis unit EDR-Z/10-1.0. Experiment were carried out on real mine water from Jeremenko water pit complex (DIAMO s.p., ODRA Ostrava branch, Czech Republic). These mine water greatly affect surface water quality, especially high concentration of dissolved solids, sulfates and chlorides. Aim of research was to treatment of mine water to quality corresponding with legislative limits for surface water. Electrodialysis tests were conducted in the batch and feed-and-bleed modes. It was demonstrated that electordialysis is good way for mine water treatment.