In practical work, implementation of the technology of aquifer thermal energy storage(ATES) is divided into energy storage phase and energy utilization phase. Sufficient cold/warm water is stored in energy storage phase, and the stored cold/warm water is consumed in energy utilization phase, so as to achieve the purpose of cooling or heating. In this paper, taking Sanhejian Coal Mine as an example, we analyze the effect of cold energy storage in multi-wells by analyzing the volume change of cold water body within different temperature ranges in different periods. Through the analysis of volume change of cold water body, it can prove in the cooling process, all of the 2-5°C cold water body is consumed, and then the 5-10°C cold water body is consumed. The volume of 10-15°C cold water body is stable, because with the consumption of colder water, part of low temperature water body changes into high temperature water body, adding the 10-15°C cold water body in aquifers. And in condition 1, there are almost the same volume of 2-5°C, 2-10°C and 2-15 °C cold water in the four cold energy storage wells.The running of 1-1’ wells, 2-2’ wells, 3-3’ wells and 4-4’ wells by sequence, all of the 2-5°C cold water body is consumed, and the 5-10°C cold water body is the mainly cold water body for cooling, and the consumption of 10-15°C cold water body is small. It proves that the cooling wells normally run, and the cold water body for cooling is sufficient, which can meet the need of cooling.