For engineering research, ECOSUNIDE technique was applied in a sewage treatment plant under low temperature and low carbon resource. The whole experimental system has being running steadily after reasonable adjustments of influent distribution, returned sludge ratio and DO. The effluent qualities meet the Class I-B Criteria Specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB18918-2002).Effluent BOD5 and NH3-N meet the Class I-A Criteria. The results indicates that the treatment capacity could fluctuate within a range of ±3000 m3/d with DO properly controlled. In aerobic sections, the best DO was 2-3 mg/L. The optimal sludge emission amount could be attained through settlement ratio and sludge concentration with the present emission amount of sludge of 680 m3/d. It might be the restrictive factor that BOD/TN was less than 3 on TN removal which could not steadily achieve the Class I-A Criteria.