Paper Title:
Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin

Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.

Chapter 1: Industry Technology, Material Engineering and Dynamic System
Edited by
Helen Zhang and David Jin
Guo Ying Fan et al., 2012, Advanced Materials Research, 459, 54
Online since
January 2012
US$ 28,-
Authors: Jin Qing Jiang, Hai Tang Zhang, Heng Zhang Zhao, Kun Zhao, Zhi Yong Xu, Xin Yao Chang, Zi Liang Wang
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Chapter 3: Material Science, Manufacturing Engineering and Production System
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Chapter 1: Industry Technology, Material Engineering and Dynamic System
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Chapter 1: Advanced Material Engineering and Dynamic System
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Chapter 1: Environmental Chemistry and Biology
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