Paper Title:
Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin

Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.

Chapter 1: Industry Technology, Material Engineering and Dynamic System
Edited by
Helen Zhang and David Jin
G. Y. Fan, J. Q. Jiang, "Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin", Advanced Materials Research, Vol 459, pp. 54-57, Jan. 2012
Online since
January 2012
US$ 28,-
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