Paper Title:
Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin
  Abstract

Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.

  Info
Periodical
Chapter
Chapter 1: Industry Technology, Material Engineering and Dynamic System
Edited by
Helen Zhang and David Jin
Pages
54-57
DOI
10.4028/www.scientific.net/AMR.459.54
Citation
G. Y. Fan, J. Q. Jiang, "Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin", Advanced Materials Research, Vol. 459, pp. 54-57, Jan. 2012
Online since
January 2012
Price
US$ 28,-
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