Paper Title:

Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin

Periodical Advanced Materials Research (Volume 459)
Main Theme Advanced Research on Industry, Information System and Material Engineering, IISME2012
Chapter Chapter 1: Industry Technology, Material Engineering and Dynamic System
Edited by Helen Zhang and David Jin
Pages 54-57
DOI 10.4028/www.scientific.net/AMR.459.54
Citation Guo Ying Fan et al., 2012, Advanced Materials Research, 459, 54
Online since January 2012
Authors Guo Ying Fan, Jin Qing Jiang
Keywords Hybridoma, Immunological Trait, Monoclonal Antibody, Sarafloxacin
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Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.