Paper Title:
Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin

Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.

Chapter 1: Industry Technology, Material Engineering and Dynamic System
Edited by
Helen Zhang and David Jin
G. Y. Fan, J. Q. Jiang, "Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin", Advanced Materials Research, Vol. 459, pp. 54-57, 2012
Online since
January 2012
Authors: Jin Qing Jiang, Hai Tang Zhang, Heng Zhang Zhao, Kun Zhao, Zhi Yong Xu, Xin Yao Chang, Zi Liang Wang
Abstract:This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against enrofloxacin (ENR) through cell fusion...
Authors: Shi Xiu Wu, Jin Qing Jiang
Chapter 3: Material Science, Manufacturing Engineering and Production System
Abstract:This paper presents an indirect competitive enzyme-linked immunosorbent assay (icELISA) using anti-mouse polyclonal antibody for rapid,...
Authors: Guo Ying Fan, Jin Qing Jiang
Chapter 1: Industry Technology, Material Engineering and Dynamic System
Abstract:A rapid indirect competitive ELISA (icELISA) for the determination of enrofloxacin (ENR) residue has been developed. EDC method was employed...
Authors: Chao Ying Li, Jin Qing Jiang
Chapter 1: Advanced Material Engineering and Dynamic System
Abstract:This study aimed to develop a monoclonal antibody based icELISA method for Ractopamine (Rac) residue. For this purpose, mixed anhydride...
Authors: Li Xin Zhu, Yan Fan, Xue Mei Qiu, Ren Rong Liu, Long Xu, Ling Xu
Chapter 1: Environmental Chemistry and Biology
Abstract:AE (Acridine ester) artificial antigens were obtained by acridine ester coupled with KLH (keyhole limpet hemocyanin) and BSA (bovine serum...