Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin
| Periodical | Advanced Materials Research (Volume 459) |
|---|---|
| Main Theme | Advanced Research on Industry, Information System and Material Engineering |
| Edited by | Helen Zhang and David Jin |
| Pages | 54-57 |
| DOI | 10.4028/www.scientific.net/AMR.459.54 |
| Citation | Guo Ying Fan et al., 2012, Advanced Materials Research, 459, 54 |
| Online since | January, 2012 |
| Authors | Guo Ying Fan, Jin Qing Jiang |
| Keywords | Hybridoma, Immunological Trait, Monoclonal Antibody, Sarafloxacin |
| Price | US$ 28,- |
Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.
