Paper Title:
Activation of MAPK and NF-κB Induced by Biomaterials in Vascular Endothelial Cells
  Abstract

Materials used to make artificial vessels and hearts can cause thrombosis, thus hindering the development of these therapeutic aids. Endothelial cells (ECs) play a key role in thrombosis, so in this study, we used human umbilical vein endothelial cells (HUVEC) and tested the hemocompatibility of different biomaterials. We then measured the signal proteins MAPK (mitogen-activated protein kinase) and Nuclear Factor- kB (NF-κB) to investigate the correlation between their expression and the degree of hemocompatibility. The results showed that ECs in the polyurethane (PU-60) group had significantly more NF-κB than the negative control (p<0.5). To identify whether the increased expression of NF-κB was induced by MAPK activation, we investigated changes in phosphorylated MAPK. We found both MAPK and NF-κB are ideal indicators for evaluating the hemocompatibility of biomaterial.

  Info
Periodical
Advanced Materials Research (Volumes 47-50)
Edited by
Alan K.T. Lau, J. Lu, Vijay K. Varadan, F.K. Chang, J.P. Tu and P.M. Lam
Pages
1335-1338
DOI
10.4028/www.scientific.net/AMR.47-50.1335
Citation
J. Sun, X. N. Mu, T. T. Ding, "Activation of MAPK and NF-κB Induced by Biomaterials in Vascular Endothelial Cells", Advanced Materials Research, Vols. 47-50, pp. 1335-1338, 2008
Online since
June 2008
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Zhen Yi Shao, An Sha Zhao, Ping Yang, Hong Fang Zhou, Nan Huang
Abstract:Lactate dehydrogenase (LDH) and enzyme-linked immunosorbent assay (ELISA) have attracted much attention recently for the evaluation of blood...
841
Authors: Ting Ting Ding, Jiao Sun, Ping Zhang
Abstract:The study of inflammation is important in understanding the reaction between biomaterials and the immune system. In the current study, human...
1423
Authors: Ying Gong, Guang Ting Han, Xin Ling Li, Yan Wu, Yuan Ming Zhang, Yan Zhi Xia, Chang Qing Yue, Da Wei Wu
Abstract:The cytotoxicity and anti-influenza virus (IFV) activity of calcium or zinc alginate fibers were investigated to explore the feasibility of...
1475
Authors: Ji Hun Seo, Mitsuhi Hirata, Sachiro Kakinoki, Tetsuji Yamaoka, Nobuhiko Yui
Chapter 2: Tissue Engineering
Abstract:The effect of molecular mobility of the supramolecular surfaces on the intracellular signaling pathway and the downstream cell functions were...
37