A novel composite electrode-containing gold/platinum nanoparticles on the vertically aligned multi-wall nanotubes (MWCNTs) by electron beam evaporation (e-beam evaporation) is reported herein. The gold/platinum nanoparticles were less than 10 nm, and distributed uniformly on the surface of vertically aligned MWCNTs under the control of e-beam evaporation. The composite electrodes could be applied in the field of biomedical sensor and energy storage devices. This process was straightforward and contained high specific surface areas of gold/platinum nanoparticles on the MWCNTs. It was helpful to enhance the efficiency of organic fuel decomposition as well as advance the sensor precision. In addition, the nanoparticles on the MWCNTs could also facilitate the high electron mobility and chemical stability.