Calcium sulfate used in this study is newly fabricated to overcome its disadvantages; brittleness, difficult handling properties, rapid resorption and reaction heating. Especially when calcium sulfate is used in the graft procedure, it is difficult to control the setting time, solubility into blood and body fluid. To improve the handling properties, Calcium sulfate is mixed with Carboxylmethylcellulose (CMS). The putty type calcium sulfate used in the present study presented lower values in cementum formation and new bone formation than the control group. This may be due to use of poor osteoinductive calcium sulfate alone in the defect, limiting new bone formation. In addition, complete resorption of calcium sulfate after 8 weeks presents that addition of polymer failed to delay the resorption rate of calcium sulfate. Rather, it seemed to disturb the natural healing of the defect when compared with the findings in the control group. But the favorable result of calcium sulfate is shown when used with alloplast. The material used in the present study was developed for easier formability and manipulation avoiding scattering or dissolving of the material with blood or body fluid. However, the results reveal that further studies on effect of calcium sulfate with various other bone graft materials and ideal ratios in mixing the two should be determined in the future.However, the results reveal that further studies on effect of calcium sulfate with various other bone graft materials and ideal ratios in mixing the two should be determined in the future.