Effect of N+ Ion Beam Exposure on the Secondary Structure of Horseradish Peroxidase
|Periodical||Advanced Materials Research (Volumes 503 - 504)|
|Main Theme||Frontiers of Manufacturing Science and Measuring Technology II|
|Edited by||Wen-Pei Sung, Jimmy Chih-Ming Kao and Ran Chen|
|Citation||Qiang Xu, 2012, Advanced Materials Research, 503-504, 705|
|Online since||April, 2012|
|Keywords||Activity, Horseradish Peroxidase (HRP), N+ Ion Beam, Secondary Structure|
After the horseradish peroxidase (HRP) was treated with different dose of N+ ion beam, the effect of N+ ion beam on the secondary structure of HRP was studied by FTIR(Fourier-transformation infrared spectroscopy). The results have shown that different dose ranging from 1015 to 1016 ions/cm2 has different effects on the secondary structure contents the HRP. The N+ implantation induce the mutual change in the secondary structure unit of HRP. It was suggested that N+ ion beam was bound to result in conformational change.