Accurate Localization of the Mobile Genomic Islands in Pseudomonas putida
| Periodical | Advanced Materials Research (Volumes 518 - 523) |
|---|---|
| Main Theme | Advances in Environmental Science and Engineering |
| Edited by | Reza Iranpour, Ji Zhao, Aijie Wang, Fenglin Yang and Xinyong Li |
| Pages | 3-7 |
| DOI | 10.4028/www.scientific.net/AMR.518-523.3 |
| Citation | Lei Song et al., 2012, Advanced Materials Research, 518-523, 3 |
| Online since | May, 2012 |
| Authors | Lei Song, Xue Hong Zhang |
| Keywords | Accurate Localization, Genomic Island (GI), Integrase, Mobility, Pseudomonas Putida |
| Price | US$ 28,- |
Pseudomonas putida is a safety gammaproteobacterium that plays an important role in bioremediation. Twenty nine mobile genomic islands were accurately localized in four strains of P. putida, six in P. putida F1, six in P. putida GB-1, nine in P. putida KT2440, and eight in P. putida W619, respectively. The integration sites include the tRNA gene, such as tRNAMet gene, tRNASer gene, tRNALeu gene, tRNAGly gene, tRNAThr gene, tRNACys gene, tRNAPro gene, and some structural genes, such as arsenate reductase gene, DNA mismatch repair protein MutS gene, thymidylate synthase gene, and 6-pyruvoyl tetrahydropterin synthase gene. 6-pyruvoyl tetrahydropterin synthase gene was firstly determined as the integration site of the genomic islands. The action sites of the lambda integrases are the stem-loop sequence, and the action sites of the P4 integrase are the asymmetric sequence. KT2440GI-5 can produce R2-type pyocin particle that is a bacteriocin and can kill sensitive bacterium. KT2440GI-9 can code ectoine-induced proteins that cause the cells to survive in high salt concentration.
