Molecular Cloning and Characterization Analysis of Phosphofructokinase Gene from Dunaliella parva
| Periodical | Advanced Materials Research (Volumes 518 - 523) |
|---|---|
| Main Theme | Advances in Environmental Science and Engineering |
| Edited by | Reza Iranpour, Ji Zhao, Aijie Wang, Fenglin Yang and Xinyong Li |
| Pages | 73-76 |
| DOI | 10.4028/www.scientific.net/AMR.518-523.73 |
| Citation | Chang Hua Shang et al., 2012, Advanced Materials Research, 518-523, 73 |
| Online since | May, 2012 |
| Authors | Chang Hua Shang, Zhong Ming Wang, Shun Ni Zhu, Lei Qin, Zhen Hong Yuan |
| Keywords | Dunaliella Parva, Molecular Cloning, Phosphofructokinase, Sequence Analysis |
| Price | US$ 28,- |
Phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway. The former studies indicated the PFK could enhance glycolysis. The full-length cDNA encoding PFK was obtained from oleaginous microalgae Dunaliella parva, which include 1572 bp open reading frame (ORF), 254 bp 5′-untranslated sequence and 323 bp 3′-untranslated sequence. Dunaliella parva PFK showed the highest sequence similarity with the PFK from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva PFK also showed wide similarity with other species.