Paper Title:

Molecular Cloning and Characterization Analysis of Phosphofructokinase Gene from Dunaliella parva

Periodical Advanced Materials Research (Volumes 518 - 523)
Main Theme Advances in Environmental Science and Engineering
Edited by Reza Iranpour, Ji Zhao, Aijie Wang, Fenglin Yang and Xinyong Li
Pages 73-76
DOI 10.4028/www.scientific.net/AMR.518-523.73
Citation Chang Hua Shang et al., 2012, Advanced Materials Research, 518-523, 73
Online since May, 2012
Authors Chang Hua Shang, Zhong Ming Wang, Shun Ni Zhu, Lei Qin, Zhen Hong Yuan
Keywords Dunaliella Parva, Molecular Cloning, Phosphofructokinase, Sequence Analysis
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Abstract

Phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway. The former studies indicated the PFK could enhance glycolysis. The full-length cDNA encoding PFK was obtained from oleaginous microalgae Dunaliella parva, which include 1572 bp open reading frame (ORF), 254 bp 5′-untranslated sequence and 323 bp 3′-untranslated sequence. Dunaliella parva PFK showed the highest sequence similarity with the PFK from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva PFK also showed wide similarity with other species.