Paper Title:
Analysis of Sulfur Metasecretome in Mixed Cultures of Acidithiobacillus Thiooxidans and Acidithiobacillus Ferrooxidans
  Abstract

The role of biomolecules in bioleaching of copper sulphide minerals carried out by bacterial consortia with predominating acidithiobacilli species is of outmost interest. The proteomic analysis on bioleaching bacterial strains have been focused up to date on full Acidithiobacillus ferrooxidans proteome, allowing the identification of proteins belonging to the general stress response, phosphate limiting conditions and the ones linked to the periplasmic fraction. Our study shows for the first time the differential expression of secreted proteins by means of standard proteomics between pure cultures of Acidithiobacillus thiooxidans and in mixture with A. ferroxidans (metasecretome) grown in sulfur, where a set of proteins is de novo synthesized in the mixed culture, identifying an Omp40-like protein possibly related to bacterial adhesion, an hypothetical protein PSEEN2944 with unknown function and up-regulation of a cytochrome c biogenesis protein, findings that give an insight into the role of proteins at the sulfur – bacteria interface, highlighting the outputs of bacterial interactions in biomining environments at the protein secretion level.

  Info
Periodical
Advanced Materials Research (Volumes 71-73)
Edited by
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
Pages
151-154
DOI
10.4028/www.scientific.net/AMR.71-73.151
Citation
R.A. Bobadilla Fazzini, P. Parada Valdecantos, "Analysis of Sulfur Metasecretome in Mixed Cultures of Acidithiobacillus Thiooxidans and Acidithiobacillus Ferrooxidans", Advanced Materials Research, Vols. 71-73, pp. 151-154, 2009
Online since
May 2009
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$32.00
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