Paper Title:
Chromosomal Arsenic Resistance Genes from Sulfobacillus Thermosulfidooxidans and a Demonstration that the Genetic Diversity of arsB among the Sulfobacilli is Similar to that of their 16S rRNA Genes
  Abstract

Arsenic resistance genes were isolated from the moderately thermophilic, Gram-positive iron and sulfur-oxidizing bacterium, Sulfobacillus thermosulfidooxidans. Only arsR and arsB genes were present and attempts to identify an arsC using degenerate PCR primers or dependent arsC genes as probes in Southern hybridization experiments were unsuccessful. Although enhanced resistance to arsenite was not detected when the ars genes were cloned in Escherichia coli, the kumamolisin-As and arsRB genes were induced by arsenite. RT-PCR experiments suggested that transcription of the cloned kumamolisin-As-like and arsRB genes is linked in Escherichia coli, but not in Sb. thermosulfidooxidans. The gene order kumamolisin-As precursor, arsR and arsB was maintained among three strains of Sb. thermosulfidooxidans isolated from three continents. Southern hybridization using a Sb. thermosulfidooxidans arsB gene fragment as a probe gave a positive hybridization signal using S. acidophilus but not with S. thermotolerans genomic DNA. Comparison of partial sequence data of the arsB and 16S rRNA genes suggested that the two types of genes have undergone a similar evolutionary history and therefore that the arsB genes were present in the ancestral Sulfobacillus before its divergence into species.

  Info
Periodical
Advanced Materials Research (Volumes 71-73)
Edited by
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
Pages
171-174
DOI
10.4028/www.scientific.net/AMR.71-73.171
Citation
J. A. van der Merwe, S. M. Deane, D. E. Rawlings, "Chromosomal Arsenic Resistance Genes from Sulfobacillus Thermosulfidooxidans and a Demonstration that the Genetic Diversity of arsB among the Sulfobacilli is Similar to that of their 16S rRNA Genes", Advanced Materials Research, Vols. 71-73, pp. 171-174, 2009
Online since
May 2009
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