Paper Title:
Predicted CO/CO2 Fixation in Ferroplasma spp. via a Novel Chimaeric Pathway
  Abstract

Previous physiological studies of the genus Ferroplasma have indicated that these microorganisms are capable of fixing CO2 in the presence of ferrous iron and low concentrations of yeast extract. Analysis of the gene complement of Ferroplasma acidarmanus fer1 and two partial genomes of Ferroplasma type I and II derived from the Iron Mountain acid mine drainage metagenome revealed the absence of several functional marker genes encoding key enzymes of three know alternative CO2 fixation routes present in archaea, i.e. the 3-hydroxypropionate cycle, the Ljungdahl–Wood pathway and the reverse TCA cycle. It is thus intriguing how these chemoautotrophic archaeal species deal with their requirements for carbon and suggests that they might have a distinct CO2 fixation route, as yet unreported. Using comparative genomics and metabolic reconstruction strategies, a putative pathway was detected for C1 fixation consisting of four main steps: 1) conversion of carbon monoxide to carbon dioxide with gain of energy and/or 2) reduction of carbon dioxide to formate, 3) incorporation of formate to tetrahydrofolate and 4) donation of the carbon moiety of tetrahydrofolate to glycine to produce serine. Steps 1 to 3 involve enzymes that correspond to some of the Ljungdahl–Wood pathway proteins, whereas step 4 resembles the well known “serine cycle”, utilized by methylotrophic microorganisms for formaldehyde fixation. Thus, this chimaeric pathway might represent the missing carbon fixation route in Ferroplasmatales. Herein, we discuss the implications of these findings in the context of central carbon metabolism requirements for biomass production in acidic environments.

  Info
Periodical
Advanced Materials Research (Volumes 71-73)
Edited by
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
Pages
219-222
DOI
10.4028/www.scientific.net/AMR.71-73.219
Citation
J. P. Cárdenas, V. Martínez, P. Covarrubias, D. S. Holmes, R. Quatrini, "Predicted CO/CO2 Fixation in Ferroplasma spp. via a Novel Chimaeric Pathway", Advanced Materials Research, Vols. 71-73, pp. 219-222, 2009
Online since
May 2009
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Fung Fei Chen, Seng Cho Chou, Hsiao Wei Chen
Chapter 5: Environmental Sciences and Engineering. GIS
Abstract:The impact of climate change and the pressure of environmental awareness have caused the concepts of energy conservation, carbon reduction,...
1478
Authors: Xiang Hui Qi, Yan Luo, Jing Fei Zhu, Huan Huan Zhang, Xu Wang, Jing Lin, Fang Chen, Zhao Ju, Liang Wang
Chapter 2: Biotechnologies and Biomedical Engineering
Abstract:Xylitol is the first rare sugar that has global market because of its excellent properties. Considering its superiority to chemosynthesis,...
149
Authors: C. Falagán, F.J. Sánchez-España, Iñaki Yusta, D. Barrie Johnson
Chapter 1: Geomicrobiology, Biogeochemical Cycles, Genetics and Molecular Biology
Abstract:Cueva de la Mora and Guadiana are two acidic mine pit lake in the Iberian Pyrite Belt (south-west Spain) that exhibit depth-related...
7