The authors have proposed a new coolant named “micro bubble coolant” in which micro bubbles (20-50µm in diameter) are included. In the previous study, it was clarified that the tool life is improved by applying this new coolant to various machining such as boring, turning and grinding. This paper deals with purification effect of the micro bubble coolant. It has been found from the experiments that bacteria which cause putrefaction of the conventional water soluble coolant are eliminated by generating micro bubbles in the coolant. It has also been clarified that the micro bubble coolant isolates minute machined chips and machine oil from the coolant.