The analyses of concrete from a bridge in Handan district shows that most bridges concrete should have cracked. The study of the technical measures against the performance deterioration of existing reinforced concrete bridges will undoubtedly become an imperative issue. In recent years, lots of bridges have been built in Handan. We choose a reinforced concrete bridge to investigate the effect of the effective member stiffness degradation and durability degradation induced by vehicle overload, vehicle overflow, rebar corrosion, and concrete deterioration on highway reinforced concrete bridges. And Static loading test was conducted at its middle span. Based on the test data in different circumstances, the seriousness of vehicle overload is discussed. In consequence, the performance of highway reinforced concrete bridges on stiffness degradation and durability degradation is more serious compared with the bridge under the condition of non-overloading. The results show that the process of rebar corrosion in highway reinforced concrete bridges is sped up under the condition of vehicle overload and vehicle overflow on the highway reinforced concrete bridges, which decreases the durability of the bridge structure, and the speed of the durability degradation increases as time goes on. The paper conclusion is instructive for the construction and maintenance of bridge.