The anti-static and anti-radiation properties of functional fabrics containing stainless steel microfilament were investigated in this paper. Three specifications of stainless steel microfilaments( diameter: 0.035mm、0.040mm and 0.050mm ) and cotton yarn were twist into composite yarns. The functional fabrics were woven by cotton yarn as warp, both cotton and the composite yarn as weft. The fabric performances were tested after woven and some treatments, especially the anti-static and anti-radiation properties. The results showed that with stainless steel microfilament increases in fabric, the amount of electrostatic charge on fabric becomes smaller. The results also showed that these fabrics had a good anti-radiation ability. So these functional fabrics have good application prospects in protective clothing and technical textiles.