Paper Title:
Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens
  Abstract

Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles (FSNP) combined with antibody molecules reported to detect pathogen detection. In this study, silica nanoparticles were prepared using the water-in-oil (w/o) microemulsion method. The silica nanoparticles were circular in diameter of 50 ± 4.2 nm. The organic dye, tris-2, 2' -bipyridyl dichlororuthenium (II) hexahydrate (Rubpy), could be incorporated efficiently into the core of silica nanoparticles. The fluorescence of Rubpy-doped silica nanoparticles was photostable using a collisional quenching fluorescence test. The Rubpy-doped silica nanoparticles were conjugated with the secondary antibody of goat anti-rabbit immunoglobulin G (IgG) and successfully detected plant pathogen such as Xanthomonas axonopodis pv. vesicatoria that causes bacterial spot disease in Solanaceae plant. These results demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.

  Info
Periodical
Advanced Materials Research (Volumes 79-82)
Edited by
Yansheng Yin and Xin Wang
Pages
513-516
DOI
10.4028/www.scientific.net/AMR.79-82.513
Citation
K. S. Yao, S.J. Li, K.C. Tzeng, T. C. Cheng, C. Y. Chang, C.Y. Chiu, C.Y. Liao, J.J. Hsu, Z.P. Lin, "Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens", Advanced Materials Research, Vols. 79-82, pp. 513-516, 2009
Online since
August 2009
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yong Qiang Zhao, Hong Lin, Lai Hao Li, Xian Qing Yang, Shu Xian Hao, Jian Wei Cen, Ya Wei, Bo Qi, Hui Huang
Chapter 3: Chemical Engineering and Materials
Abstract:A novel Schiff base, 2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence...
1483
Authors: Ming Tian Li, Rui Song Yang, Lan Ying Yu
Chapter 3: Material Application, Green Chemistry, and Mining Engineering
Abstract:To detect noradrenaline (NA) concentration, a fiber optic biosensor based on CuPc catalysis and fluorescence quenching was fabricated and...
439
Authors: Yu Fen Liu, Hai Tao Xia, De Fu Rong
Chapter 10: Chemical Biology and Medicinal Chemistry
Abstract:The binding reaction of Zn(II) complex [Zn(C8H10N)2Cl2] with bovine serum albumin(BSA) was...
1678
Authors: Zakaria Nor Dyana, Abdul Razak Khairunisak, Abdul Aziz Azlan
Chapter 5: Ceramic Materials
Abstract:Amorphous nanosilica entrapped fluorescence dye intended to be used as tracing agent for imaging of cell or tissue in human cell was prepared...
285
Authors: Zhi Liang Jiang, Mei Ling Tang, Qing Ye Liu, Ai Hui Liang
Chapter 1: Materials Engineering. Technologies and Processing
Abstract:In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA)...
18