In natural seawater environment, temperature and chlorine ion are important factors for organic coating degradation. The alternating pressure of seawater can also affect the lifetime in deep-sea environment and probably is conducive to synergistic effects with other ageing factors. The present work is to investigate the effect of alternating pressure of seawater on protective properties of epoxy organic coating on 907A steel substrate, and evaluates the organic coating degradation process after pressure tests at atmospheric pressure. EIS was used to study the degradation process of the organic coating in natural seawater. The results demonstrated that the impedance of coating under alternating pressure of seawater conditions decreased faster than that at atmospheric pressure. With the increase of alternating pressure cycles, the entire degradation process of organic coating was accelerated. It is concluded that alternating pressure of seawater has to be considered as an aggressive factor like temperature or chlorine ion.