Distiller's Dried Grains with Solubles (DDGS) is used to replace starch as a major feedstock material to produce bio-adhesive. The experimental conditions and the preparation process of the DDGS bioadhesive are outlined. The production and performance of DDGS adhesives were directly influenced by the heating temperature, heating time and concentration of hydroxide (NaOH) solution. When the heating temperature was 90 °C, the heating time was 10 min, and the concentration of NaOH solution was 30g/L, the yield of the DDGS adhesive was 61.6% wt/dry wt. The DDGS adhesive was less sensitivity to humidity than commercial starch adhesive.