Paper Title:
Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering
  Abstract

Nature is full of many interesting things to work with, but many natural resources are also protected. In this view the recycling of aquaculture and fishery residues may lead to the manufacture of new devices and the isolation of new molecules with potential application in medicine. The aim of the present study was to explore the possibility to transform the cuttlefish bone into an hydroxyapatite scaffold suitable for bone tissue engineering application. The mixture of different lamellar porous structure of cuttlefish bone from the species Sepia Officinalis was selected and characterized, according to morphology (including porosity, surface development, surface characteristics) and mechanical properties. The material was transformed into suitable scaffold for bone tissue regeneration, trying to totally or partially convert calcium carbonate (aragonite) into calcium phosphate (hydroxyapatite HA) using hydrothermal transformation. The studies on cell attachment and proliferation (by MTT assay at different experimental times), cell morphology with Scanning Electron Microscopy (SEM), alkaline phosphatase (ALP) and osteocalcin (OC) activities and expressions by mouse osteoblast-like MC3T3-E1 cells on HA were investigated at different experimental times in cultures, in comparison with those observed on titanium specimens used as a control (ET and ST). Cell proliferation was less in HA transformed cuttlefish bone scaffolds than in ET and ST specimens. In contrast, good performance for osteoblasts differentiation was observed on HA transformed cuttlefish bone scaffolds, similar to those observed onto titanium scaffolds.

  Info
Periodical
Advanced Materials Research (Volumes 89-91)
Edited by
T. Chandra, N. Wanderka, W. Reimers , M. Ionescu
Pages
47-52
DOI
10.4028/www.scientific.net/AMR.89-91.47
Citation
E. Battistella, S. Mele, S. Pietronave, I. Foltran, G.I. Lesci, E. Foresti, N. Roveri, L. Rimondini, "Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering", Advanced Materials Research, Vols. 89-91, pp. 47-52, 2010
Online since
January 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jun Yao, Shula Radin, Gwendolen Reilly, Phoebe S. Leboy, Paul Ducheyne
Abstract:A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the...
619
Authors: S.J. Heo, S.E. Kim, Yong Taek Hyun, D.H. Kim, Hyang Mi Lee, Yeong Maw Hwang, S.A. Park, Jung Woog Shin
Abstract:This study evaluated the potential of the PCL (poly -caprolactone)/HA(Hydroxyapatite) composite materials as a scaffold for bone...
369
Authors: M.T. Rodrigues, Manuela E. Gomes, João F. Mano, Rui L. Reis
Abstract:Bone marrow cells are a potential source to induce different lineage cells which can be used to rebuild or replace damaged tissues using a...
72
Authors: Frank Lüthen, Claudia Bergemann, Ulrike Bulnheim, Cornelia Prinz, Hans Georg Neumann, Andreas Podbielski, Rainer Bader, Joachim Rychly
Abstract:To stimulate bone regeneration, the design of bioactive implants is a great challenge in current orthopedic research. We reasoned that...
600
Authors: Nuttapon Vachiraroj, Siriporn Damrongsakkul, Sorada Kanokpanont
Abstract:In this work, we developed a 3-dimensional bone tissue engineering scaffold from type B gelatin and hydroxyapatite. Two types of scaffolds,...
121