In the present study, nucleation at Cube bands during annealing of a cold rolled commercial aluminium alloy AA8079 was investigated. The main goal of this work was to provide experimental data for subsequent modelling. By studying the deformed material the geometrical parameters of Cube bands were determined. The Cube band spacing in the deformed state was measured experimentally and accordingly calculated with a simple geometric model, taking the initial Cube fraction in the pre-deformed state and the deformation degree into account. The nucleation frequency during subsequent annealing of the cold deformed material was estimated from the geometry and size of deformed Cube bands prior to deformation. From investigations of the orientation relationship between the Cube oriented nuclei and the adjacent deformed matrix with S orientation, the fraction of 40°<111> grain boundaries having optimum growth conditions, was established.