The synthesis of cellulosic plastic films from waste cotton fabrics under homogeneous conditions by microwave heating using lauroyl chloride as an esterifying agent was studied. Nevertheless, the cellulose laurate film obtained was quite brittle than some other commercial synthetic polymers. Thus, the aim of this work was to investigate the effects of plasticizer on the properties of cellulose laurate film. Cellulose laurate was plasticized with each plasticizer, triethyl citrate (TEC), dibutyl phthalate (DBP), and epoxidized soy bean oil (ESO) at different amounts. The content of plasticizer was varied from 3% to 9% w/w of dry cellulose laurate. The effects of plasticizer (i.e., type and content) on mechanical properties of plasticized cellulose laurate films were investigated. The morphology and structure of cellulose laurate were confirmed by SEM, FTIR, and 1H-NMR analysis, whereas the thermal properties were characterized by TGA.