Paper Title:
Effect of Thai Silk Sericin and its Extraction Methods on L929 Mouse Fibroblast Cell Viability
  Abstract

Sericin, one of the two main proteins of silk cocoon besides fibroin, has been wildly used as ingredient in cosmetic products and nutrition supplements; however there are considerable controversial reports on its toxicity to cells and its advantages. This work aimed to investigate cell biocompatibility of sericin both in the systems of blended silk fibroin/sericin and pure sericin films using L929 mouse fibroblasts. The effect of concentration of commercial heat extracted sericin on cell viability was first investigated in the system of silk fibroin/sericin (F/S) films using 2 types of silk fibroin, Bombyx mori Nangnoi Srisaket 1 and Bombyx mori Jul 1/1. For both types of silk fibroin, it was found the lower cell number attached and proliferated on the blended F/S films at all sericin concentrations, compared to that on glass and pure fibroin. However, proliferation rate of cells cultured on the blended F/S films was similar to that of cells cultured on glass and pure fibroin films, as confirmed by population doubling time (PDT). Cytotoxicity of sericin extracted from 4 different methods including heat, acid, alkali and urea treatments was further studied in this work in the system of pure sericin films. It could be seen that acid and urea extracted sericin films showed high percentage of cell attachment at 92% and 88%, respectively. However, number of cells proliferated on all sericin films after 48 h culture was not significantly different. This indicated that L929 cells had different proliferation rate when cultured on different types of sericin films. Among 4 extraction methods, the PDT of cells proliferated on urea extracted sericin film was lowest (27 h) and also lower than that of the blended F/S films. This study suggested that sericin extracted by urea treatment could enhance proliferation rate of L929 mouse fibroblasts.

  Info
Periodical
Advanced Materials Research (Volumes 93-94)
Edited by
S. Suttiruengwong and W. Sricharussin
Pages
385-388
DOI
10.4028/www.scientific.net/AMR.93-94.385
Citation
P. Thitiwuthikiat, P. Aramwit, S. Kanokpanont, "Effect of Thai Silk Sericin and its Extraction Methods on L929 Mouse Fibroblast Cell Viability", Advanced Materials Research, Vols. 93-94, pp. 385-388, 2010
Online since
January 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Rui Juan Xie, Ming Zhong Li, Shen Zhou Lu, Wei Hua Sheng, Yu Feng Xie
Abstract:Bombyx mori Sericin films were prepared with PEG-DE as cross linking agent. The main physical properties, structure characteristics,...
241
Authors: Lin Li, Yi Li, Jia Shen Li, Lei Yao, Arthur F.T. Mak, Frank Ko, Ling Qin
Abstract:Poly-L-Lactide (PLLA) composites were prepared with nano silver particles with the weight ratios of nano silver particles to PLLA (Ag/PLLA)...
849
Authors: J. Chamchongkaset, Sorada Kanokpanont, David L. Kaplan, Siriporn Damrongsakkul
Abstract:Silk has been used commercially as biomedical sutures for decades. Recently silk fibroin, especially from Bombyx mori silkworm, has been...
685
Authors: Fang Zhang, Jiao Jiao Li, Rui Jiang, Shan Shan Zhang, Tian Zhu, Shen Zhou Lu
Symposium J: Biomedical Materials
Abstract:Various methods were developed to prepare hydrogels including photo-cross-linking, chemical cross-linking, enzymatic cross-linking, pH or...
407