The function integration, process integration and information integration of the manufacturing system are all in need of the support of the personnel integration. But the complicated relationship among personnel has caused difficulties in personnel integration. In order to analyze the correlation between personnel in manufacturing system, an analysis method based on the relation degree matrix was proposed, meanwhile the personnel in manufacturing system was classified according to the product life cycle(PLC). The above work has paved the path for the further development of a kind of operation mode for personnel integration in manufacturing system based on PLC (MOPI). The mode is composed of four layers, which are the target layer, the PLC layer, the personnel layer, and the supporting system layer. The above mode was implemented in a local manufacturing enterprise, and positive application effect was achieved.