This paper discusses the effect of two similar gold wire (wire A and wire B) used mainly as a wire bonding material for Quad Flat Nolead (QFN) package. Both wires with diameter of 25.4 μm were bonded using automatic wire bonder by maintaining the temperature at 200°C. The effect of trace elements on the mechanical properties of 4N gold wire has not been widely investigated for some years despite the important of wire-bonding and the move towards fine pitch applications. Due to the element analysis, atomic percentage of Ca in wire B is higher than wire A. Pull strength increase with the increasing of the trace element. The higher pull strength of wire B could improve the yield strength, elastic modulus and recrytallization temperature.