Paper Title:
Electrical Characteristics of Extended Gate FET Sensing Chip Constructed for Detection of DNA
  Abstract

An extended gate field effect transistor (EGFET) sensing chip has been constructed by using one gold plate electrode for molecule recognition and FET part for signal transduction. By using a 70.7mV DC voltage onto a Ag/AgCl reference electrode, the electrical characteristics of immobilization of the oligonucleotide probe of P1 and hybridization with the target single strand DNA of P2 on the EGFET sensing chip were examined in detail. The electrical signals on the change of a threshold voltage (VT) shift at a constant ID (3000μA) in VG-ID characteristic were obtained, and the VT shift value due to hybridization was calculated to be 12 mV, which may be attributed to the decreased negative charges after hybridization occurred at the gate surface. The surface density of hybridized dsDNA on gold surface of the FET was evaluated to be about 1 × 1012 molecules/cm2, indicating that the EGFET was a promising sensing element for biochip.

  Info
Periodical
Advanced Materials Research (Volumes 97-101)
Edited by
Zhengyi Jiang and Chunliang Zhang
Pages
4189-4192
DOI
10.4028/www.scientific.net/AMR.97-101.4189
Citation
Z. Cao, Z. L. Xiao, Y. L. Dai, M. Kamahori, M. Shimoda, "Electrical Characteristics of Extended Gate FET Sensing Chip Constructed for Detection of DNA", Advanced Materials Research, Vols. 97-101, pp. 4189-4192, 2010
Online since
March 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Tae Song Kim
Abstract:The microcantilevers have emerged as a versatile biosensor, and showed excellent performance such as high sensitivity, high selectivity, and...
337
Authors: Li Yan Zhao, Yan Fei Tian, Jiao Yun Xia, Jing Lin He, Ling Zhang, Zhong Cao
Abstract:A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of oncogene of c-Myc tagged DNA...
2935
Authors: Li Bo Nie, Hao Chang, Zhu Chen, Quan Guo He
Chapter 19: Biological Information Technology
Abstract:As the substrates of biosensor or biomicroarray, silicon, silica and gold matrixes are usually used as the surfaces of bio-recognition. In...
2991
Authors: Wen Pin Hu, Li Ya Huang, Wen Yih Chen
Chapter 1: Materials Engineering and Processing Technologies of Materials
Abstract:A stable and versatile biosensor surface is a desirable feature for any sensor, and the DNA-directed immobilization (DDI) of protein-DNA...
398
Authors: Ting Ting Gu, Hong Qi Xia, Jian Li Wang, Da Wei Yang, Yang Zhao, Mo Han Liu, Zhi Gang Fang
Abstract:Horseradish peroxidase (HRP) and toluidine blue (TB) were incorporated in polyion complex membrane composed of double stranded DNA(dsDNA) and...
84