Novel superstructures of zinc oxide thin film with nano-rods and nano-screws were prepared via soft solution route without any polymer additives. The thin film of ZnO could be produced directly on the substrates. The yield of nano-rod and/or nano-screw was almost 100%. Small amount of nano-disk superstructure was also obtained. The superstructures of the film changed depending on the kind of treatment organic solvent such as methanol, butanol, i-propanol, hexane, etc. Monodispersed or symbiotic/multipod ZnO nanorods with hexagonal cross section or screw microstructure were successfully prepared.