Bionanotechnology has seen much interest in the past few years. The development in new nanotechnologies and the transfer of such to biomedical applications has been received with large expectations. Here we will describe some of the most common techniques to prepare surfaces with nanometric sized features and how they have been applied to control cell behavior. The focus, however, will be on electron beam lithography and its use in biological applications. We will show that such highly ordered surfaces exhibit low adhesive properties for cells. Also, such topographies change the wetting properties to be either more hydrophilic or hydrophobic depending on the surface energy of the flat surface. Today, little research has found its way to the commercial market. This is mainly down to the ability to make large areas or large quantities of nano patterned materials. We will describe a few methods by which we think it would be possible to mass produce nano topographically patterned surfaces.