Paper Title:
Controlled Release of Thrombin Using Aptamer-Based Nanodevices
  Abstract

Aptamers are DNA or RNA single strands that have been selected from random pools based on their ability to bind ligands. Like antibodies, aptamers are highly specific to their targets, and thus have many potential uses in biomedicine and biotechnology. We report here on the construction of a protein-binding molecular device based on a DNA aptamer, which can be instructed to hold or release the human blood-clotting factor, α-thrombin, depending on an operator DNA sequence addressing it. In the operation of this DNA nanodevice, the thrombin-binding DNA aptamer is switched between a binding and a non-binding form. This is achieved by sequentially hybridizing and removing a DNA single strand to the protein binding region of the aptamer. This principle of operation is limited as the switching sequence is determined by the protein-binding sequence. To overcome this limitation we introduce a DNA signal translation device that allows the operation of aptamers with arbitrary sequences. The function of the translator is based on branch migration and the action of the endonuclease FokI. The modular design of the translator facilitates the adaptation of the device to various input or output sequences.

  Info
Periodical
Edited by
P. VINCENZINI and R. BARBUCCI
Pages
116-121
DOI
10.4028/www.scientific.net/AST.53.116
Citation
S. Beyer, W. U. Dittmer, A. Reuter, F. C. Simmel, "Controlled Release of Thrombin Using Aptamer-Based Nanodevices", Advances in Science and Technology, Vol. 53, pp. 116-121, 2006
Online since
October 2006
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Eike Friedrichs, Ralf Jungmann, Angeliki Tsokou, Stephan Renner, Friedrich C. Simmel
Abstract:DNA has been recently used to construct a variety of nanoscale machines and switches, among them devices which can translocate, compute, or...
120
Authors: Qing Ye Liu, Gui Qing Wen, Fang Gui Ye, Ai Hui Liang
Chapter 2: Biochemistry
Abstract:Highly sensitive and selective surface-enhanced resonance Raman scattering (SERRS) spectral detection technique are developed by combining...
145
Authors: Yan Xia Yan, Li Ying Jiang, Fen Fen Wang, Yan Zhang, Jie Hu
Chapter 2: Micro Sensors
Abstract:A new fabrication methods of thin-film gold electrode is reported. Electrochemical characteristics of self-assembly aptasensor based on MEMS...
441
Authors: Han Feng Cui, Hao Fan, Yan Lin
Chapter 13: Sensor Technology
Abstract:A sensitive electrochemical aptasensor for detection of thrombin based on target protein-induced strand displacement is presented. For this...
2113