Paper Title:
Production of Novel Exopolysaccharide with Emulsifying Ability from Marine Microorganism, Alteromonas sp. Strain 00SS11568
  Abstract

To find a novel exopolysaccharide, marine bacterial strains were isolated from coastal regions of Korea. Strain 00SS11568 was then selected as it produced a mucous exopolysaccharide during the stationary phase in a batch culture. The isolate was identified as Alteromonas sp. based on its 16S rDNA sequence, morphological, and biochemical properties. The exopolysaccharide, designated as p-11568, exhibited an emulsifying ability. The Emulsification Index (E24) of 0.1% p- 11568 was 77.4% with an emulsified kerosene content, and was higher than those of commercial polysaccharides, such as xanthan gum (26.1%), gellan gum (1.3%), and sodium alginate (2.0%). p- 11568 was found to be composed of glucose and galactose as the main natural sugars in a molar ratio of 1.3:1, along with uronic acid (18.9%, w/w) and sulfate groups (1.2% w/w). The average molecular mass was 4.4 x 105 daltons by gel filtration chromatography. The effects of pH, temperature, inorganic compounds, and C and N sources were tested to obtain the optimal medium composition for the production of p-11568. Under optimal growth conditions with the M-11568 medium, 14.9 g of crude p-11568 per liter was obtained.

  Info
Periodical
Key Engineering Materials (Volumes 277-279)
Edited by
Kwang Hwa Chung, Yong Hyeon Shin, Sue-Nie Park, Hyun Sook Cho, Soon-Ae Yoo, Byung Joo Min, Hyo-Suk Lim and Kyung Hwa Yoo
Pages
155-161
DOI
10.4028/www.scientific.net/KEM.277-279.155
Citation
J. H. Yim, S. H. Ahn, S. J. Kim, Y. K. Lee, K. J. Park, H. K. Lee, "Production of Novel Exopolysaccharide with Emulsifying Ability from Marine Microorganism, Alteromonas sp. Strain 00SS11568", Key Engineering Materials, Vols. 277-279, pp. 155-161, 2005
Online since
January 2005
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Price
$32.00
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