A different range of vancomycin was incorporated into a poly dl-lactide-co-glycolide) [PLGA] disc matrix using the hot compression method. The disc was placed in phosphate buffered saline (PBS) and incubated at 37oC. The PBS was changed periodically, and the removed solution was analyzed using high performance liquid chromatography. The sensitivity of the antibiotic-polymer matrix was evaluated using cultured-human-ear methicillin-resistant staphylococcus aureus (MRSA). The MRSA growth was inhibited proportionally to the incorporated vancomycin concentration in the disc matrix. A disc incorporating 400 µg of antibiotics inhibited the growth of MRSA 10 times longer than the disc containing 30 µg as a control. It was found that the biodegradable polymer matrix enabled the control-release of an antibiotic, and the release profile was observed as zero order.