The three different structures of titanium oxide film were prepared: (1) The commercial pure titanium was treated with heating in air at 700°C for half hour and gotten a dense rutile film on titanium (HS Samples); (2) The commercial pure titanium was treated by chemically treating and gotten a layer of amorphous titania gel on the Ti surface (TS Samples); (3) After chemically treating, the samples were heated in air at 700 °C for half hour, and gotten nano-particles coalesced microporous titanium oxide (rutile) film on titanium surface (XS sample). The dense rutile and amorphous titania gel did not induce apatite formation on their surfaces in SBF solution for 48 hours, whereas the nano-particles coalesced microporous rutile structure induced apatite formation on their surfaces. Mechanical test and histological examination were investigated after the samples implanted in dogs limbs for 3 months. The results of push-out test are 12.96, 29.48 and 35.83 MPa respectively for HS, TS and XS sample. Histological results showed that TS sample and XS sample contacted the bone directly, without any intervening fibrous tissue, and there was a fibrous tissue layer between the bone and HS samples.