Paper Title:
Comparison of Validity between WST-1 and MTT Test in Bioceramic Materials
  Abstract

Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of the extracts were measured by MTT and WST-1 assay and their pH were measured with pH meter. The cellular survival rate of CPG was the lowest and the results of the WST-1 test showed results similar to those of the MTT test. Thus, proliferation assays using WST-1 may be conveniently and routinely applicable to pre-clinical evaluation of bioceramics.

  Info
Periodical
Key Engineering Materials (Volumes 284-286)
Main Theme
Edited by
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
Pages
585-588
DOI
10.4028/www.scientific.net/KEM.284-286.585
Citation
K. M. Kim, S. B. Lee, S. H. Lee, Y. K. Lee, K. N. Kim, "Comparison of Validity between WST-1 and MTT Test in Bioceramic Materials ", Key Engineering Materials, Vols. 284-286, pp. 585-588, 2005
Online since
April 2005
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: D. Marković, V. Živojinović, V. Koković, V. Jokanović
555
Authors: Xiao Yan Lin, Hong Song Fan, Xu Dong Li, Min Tang, Ling Li Zhang, Yan Fei Tan, Jin Rui Xu, Xing Dong Zhang
Abstract:A nano-grade hydroxyapatite/collagen composite was prepared by an in situ synthesis technique from calcium nitrate, diammoniun hydrogen...
195
Authors: Li Zhao Teng, Hong Song Fan, Xing Dong Zhang
Abstract:The purpose of this study was to evaluate biocompatibility of a new injectable root canal sealer. The cytotoxicity of zinc oxide-eugenol...
1357
Authors: Deni Noviana, Sri Estuningsih, Devi Paramitha, Mokhammad Fakhrul Ulum, Hendra Hermawan
Chapter 7: Biomaterials and Biomedical Engineering
Abstract:A foreign body is any object originating outside the body. It may migrate from its entry site and cause pain, inflammation and infection....
449
Authors: Lin Wang, Shen Hua Song, Zheng Zhi Wu, Li Hong Duan, Chun Bao Wang
Chapter 6: Materials for Biomedical Application
Abstract:In order to evaluate the cytocompatibility and hemolytic properties of n-HA/PEEK biocomposites the nanohydroxyapatite/polyetheretherketone...
567