Paper Title:
Apatite Layer with Serum Protein as a Suitable Scaffold for Growth of Osteoblast-Like Cell
  Abstract

Autograft, allograft, and biomaterials had been developed for bone regeneration. In recent year, a tissue engineering technique has been paid much attention for next generation implant. A problem of bone tissue engineering to be solved is a development of the substrate that is suitable for cell adhesion, proliferation, and differentiation. A biomimic scaffold for tissue culture was proposed, and then a cell response on the scaffold was estimated. The scaffold composed by a calcium deficient apatite with an adsorbed serum protein was formed on a ceramic hydroxyapatite (HAp) and surface-modified titanium by a soaking in cell-culture medium supplemented with fetal bovine serum. Excellent results on cell proliferation and cell adhesion were obtained only on osteoblast-like cells (MC3T3-E1). An actin filament in narrow filopodium of the spindle-shaped MC3T3-E1 cells on the ceramic HAp had a regular course. On the other hand, ends of the actin filament of the widely spread cells on the apatite layer with serum protein were scattering. It was suggested that the scattering of the actin end showed an existence of fibronectin, and then tight adhesion would be obtained by the many focal adhesion. Accordingly, the effectiveness of the biomimic scaffold containing serum protein on cell growth was confirmed.

  Info
Periodical
Key Engineering Materials (Volumes 284-286)
Main Theme
Edited by
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
Pages
611-614
DOI
10.4028/www.scientific.net/KEM.284-286.611
Citation
T. Kizuki, M. Ohgaki, K. Yamashita, Y. Yokogawa, "Apatite Layer with Serum Protein as a Suitable Scaffold for Growth of Osteoblast-Like Cell", Key Engineering Materials, Vols. 284-286, pp. 611-614, 2005
Online since
April 2005
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Price
$35.00
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