Paper Title:
Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells
  Abstract

This paper demonstrated a simple technique to seed the hydroxyapatite (HA) ceramic with 2-D cultured cells to establish an advanced mode of HA grafting. The HA beads with a through-hole were fabricated to have an oblate shape to keep an opening of the through-hole contact with 2-D cultured cells layer. The seeding was performed by leaving the HA beads in a culture dish with MG63 cells in culture. The through-hole trapped MG63 cells in a sheet form by 1-day seeding. After 5 days, the number of trapped cells increased about nine fold, forming cell clumps in the through-hole. The seeding of the HA beads was considered an easy and practical method to provide HA/Cell hybrids that would realize active bone forming orthopedic treatment.

  Info
Periodical
Key Engineering Materials (Volumes 284-286)
Main Theme
Edited by
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
Pages
683-688
DOI
10.4028/www.scientific.net/KEM.284-286.683
Citation
K. Teraoka, T. Saito, Y. Yokogawa, "Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells", Key Engineering Materials, Vols. 284-286, pp. 683-688, 2005
Online since
April 2005
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Nurşen Koç, Muharrem Timuçin
2207
Authors: Mamoru Aizawa, A. Hiramoto, H. Maehashi, Tomokazu Matsuura
Abstract:We have previously developed apatite-fiber scaffolds (AFSs) for bone tissue engineering using single-crystal apatite fibers and carbon...
1165
Authors: Teruo Asaoka, Yuji Kajihata, Katsuko Furukawa, Takashi Ushida, Tetsuya Tateishi
Abstract:As excellent scaffolds for cultivating bone cells, porous beads of bioactive ceramics such as HAp, TCP are considered to be promising. HAp...
2021
Authors: Shu Shan Yao
Chapter 9: Nano Materials
Abstract:Carbon nanotubes, carbon spheres and slices of vertically aligned multiwalled carbon nanotubes (MWNTs) were synthesized simultaneously by...
1325
Authors: A. Tamura, Teruo Asaoka, K. Furukawa, T. Ushida, T. Tateishi
Chapter 3: Tissue Engineering and Regenerative Medicine
Abstract:α-TCP/HAp functionally graded porous beads were prepared for use as a scaffold for bone regeneration. In this research, porous beads which...
63