The objective of this study was to assess the behaviour of nacre pinctada margaritifera (PM) in subcutaneous site in rat and to taste the influence of sterilization on nacre properties. PM discs or powder were sterilized at 120 degree celsius in one bar of pressure during 20 minutes or with gamma ray or in alcohol (70 %) .PM discs or powder were implanted for 3, 6 and twelve weeks in subcutaneous site in rat. The methods used to assess behaviour of PM were: photonic microscopy, scaning and transmission electron microscopy and gravimetry. The results have not shown any diference between the implants. PM is biodegradable, biocompatible but did not show any osteoinductive properties.