Paper Title:
Enhanced Biological and Mechanical Properties of Artificial Dermis Prepared Using a Perfusion Culture System
  Abstract

A culture system that is capable of providing even and uniform distribution and deposition of cells and extracellular matrix (ECM) is desired to enhance biological functions of the tissue-engineered artificial dermis (TEADs). For this purpose, we have developed a perfusion culture system that offers uniform exchange of nutrients and gases along the scaffold. Viability and effectiveness of the system were investigated by comparing biological and mechanical properties of TEADs. Results showed that the TEADs constructed by the perfusion culture system revealed significantly increased cell growth, ECM synthesis, and elastic modulus compared to those by the conventional static culture system. In addition, histological findings indicated that cells were more evenly distributed and ECM deposition increased in TEADs with the perfusion culture system. Therefore, it can be suggested that the perfusion culture system can constitute a more promising approach for constructing the TEADs.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
23-26
DOI
10.4028/www.scientific.net/KEM.288-289.23
Citation
D. L. Seol, H. I. Kim, W. J. Lee, W. H. Jang, J. K. Kim, S. J. Lee, Y. I. Yang, "Enhanced Biological and Mechanical Properties of Artificial Dermis Prepared Using a Perfusion Culture System", Key Engineering Materials, Vols. 288-289, pp. 23-26, 2005
Online since
June 2005
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Price
$32.00
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