Paper Title:
The Influences of β-Glucan Associated with BMP-7 on MC3T3-E1 Proliferation and Osteogenic Differentiation
  Abstract

b-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. Biologic response modifiers, like beta-glucan, will modulate immunity, modify neoplastic disease and increase resistance to microbial challenge. Therefore, beta-glucan polymers can be applied in bone induction and regeneration model and have a possibility of association with bone morphogenetic protein (BMP) because of tissue-regenerative and antimicrobial effects of those polymers. In this report, we studied an E. coli expression system for BMP-7 production and the biological activities of b-glucan associated with BMP-7. The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium b-glucan, while neither mushroom b-glucan nor barley b-glucan increased the cell proliferation. Mushroom b-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other b-glucans did not affect ALP activity of the cells. In mineralization assay, a highly significant increase in nodular staining was observed in cultures treated with both mushroom and Aureobasidium b-glucans in the presence of BMP-7 compared with nontreated controls, while barley b-glucan showed a significant decrease in nodule number compared with cultures treated only with BMP-7.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
241-244
DOI
10.4028/www.scientific.net/KEM.288-289.241
Citation
D.H. Lee, D. W. Han, B.J. Park, H. S. Baek, K. Takatori, M. Aihara, K. Tsubaki, J. C. Park, "The Influences of β-Glucan Associated with BMP-7 on MC3T3-E1 Proliferation and Osteogenic Differentiation", Key Engineering Materials, Vols. 288-289, pp. 241-244, 2005
Online since
June 2005
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$32.00
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