Collagen-HA scaffolds were prepared by a phase-separation technique, cross-linked by formaldehyde or carbodiimide(EDC)/N-hydroxysuccinimide(NHS), which is also used to covalently attach chondroitin sulfate(CS) to the scaffold. BMP was incorporated into the scaffold by adsorption. The scaffolds were characterized by SEM and ESCA. Rat mesenchymal stem cell(MSCs) was seeded into the scaffold and cultured to form a cell/scaffold construct. The ectopic osteoinduction of the scaffolds were evaluated in vivo. The results show that the scaffolds are porous with three-dimensionality, have good cell compatibility and osteoinduction. Attachment of CS can improve the adhesion and differentiation of cells.