Paper Title:
Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films
  Abstract

In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 µg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 µg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 µg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
291-294
DOI
10.4028/www.scientific.net/KEM.288-289.291
Citation
H. J. Son, D. W. Han, H.H. Kim, H. J. Kim, I. S. Lee, J. K. Kim, J. C. Park, "Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films", Key Engineering Materials, Vols. 288-289, pp. 291-294, 2005
Online since
June 2005
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Price
$32.00
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