Paper Title:
Study of Bone Marrow Derived Stem Cells and Gene Therapy for Engineering of Cardiovascular Grafts
  Abstract

Recent study shows that endothelial progenitor cells (EPCs) and gene therapy technologies are effective strategies in the inhibition of stenosis and thrombus formation and improving the patency rate of the vascular graft in vivo. In this study, rat EPCs were cultured from bone marrow, and plated in fibronectin-coated plates with EBM-2 medium. Bone marrow mesenchymal stem cells (MSCs) were cultured with alpha minimum essential medium ( -MEM). After two weeks, EPCs were immunohistochemically characterized using antibodies specific for endothelial cells. Retroviral vectors pMSCV-eNOS, pMSCV-tPA, pMSCV-LacZ and pMCSV-GFP were constructed. Retroviral particles were produced using packaging cell line 293T cells. Gene transfer was carried out by exposing cells to virus solution for 6 hours in the presence of 8µg/ml polybrene. For constructing vessels, MSCs and EPCs were seeded on fibronectin coated ePTFE graft in tissue culture condition for 2-4 weeks. The attachment and growth of cells were analyzed with scanning electron microscopy (SEM). Our data showed that the EPCs expressed VEGF, Lectin BS-1, RECA-1, indicating they are endothelial lineage. The concentrated retroviral particles showed many folds higher transduction efficiency to NIH 3T3 cells than the commercial reagent Fugene. SEM data showed dense attachment of MSCs on the graft surface. MSCs/EPCs co-culture gave much better cell coverage on the graft than culture of EPCs alone.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
31-34
DOI
10.4028/www.scientific.net/KEM.288-289.31
Citation
J. Zhang, Y. Zhang, Y. J. Duan, L. L. Ou, Y. T. Yu, D. Kong, "Study of Bone Marrow Derived Stem Cells and Gene Therapy for Engineering of Cardiovascular Grafts", Key Engineering Materials, Vols. 288-289, pp. 31-34, 2005
Online since
June 2005
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Patrick Frayssinet, Eliane Jean, N. Rouquet
861
Authors: Ho Yun Chung, Eun Jung Oh, Jin Hyun Choi, Byung Chae Cho
Abstract:Mesenchymal stem cells (MSCs) from bone marrow seem to be the one of best candidates to regenerate injured tissue. However, recent advances...
193
Authors: Leandra S. Baptista, Carolina S.G. Pedrosa, Karina R. da Silva, Ronaldo J.F.C do Amaral, Michele C.L. Kochem, Hélio Menezes, Aris Sterodimas, José de Faria, Marcio R.R. de Oliveira, Radovan Borojevic
Abstract:Silks are naturally occurring polymers and fibroin, its filament core protein, has been shown to support stem cell differentiation in vitro,...
437
Authors: Betül Çelebi, Nicolas Pineault, D. Mantovani
Chapter 3: Biomaterials
Abstract:The three dimensional scaffold of the bone marrow (BM) niches is composed of various elements including extracellular matrix proteins and...
111
Authors: Wei Chung Liu, Chang Shu Tsai, Ya Yun Chen, Nien Tzu Keng
Chapter 2: Advanced Bio and Medical Engineering, Applications of Bioinformatics and Genetic Engineering
Abstract:Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological...
494