Effect of Fibrin/Collagen Matrix on Osteogenic Differentiation of Bone Marrow Stromal Cells |
| Journal |
Key Engineering Materials (Volumes 288 - 289) |
| Volume |
Advanced Biomaterials VI |
| Edited by |
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata |
| Pages |
35-38 |
| DOI |
10.4028/www.scientific.net/KEM.288-289.35 |
| Online since |
June, 2005 |
| Authors |
Young Il Yang,
Dong Lim Seol,
S.J. Jeong,
D.S. Lee,
Won Hee Jang,
S.S. Seo,
Sung Jae Lee
|
| Keywords |
Bone Marrow Stromal Cells, Collagen, Fibrin, Hydrogel, Osteogenic Differentiation |
| Abstract |
Fibrin is a natural polymer with excellent biocompatibility and widely used as a cell
delivery vehicle in tissue engineering. However, fibrin of low concentration is not able to promote cell growth and differentiation within a desired time because of contraction and biodegradation of cell-seeded matrices. In this study we investigated effects of combining fibrin with collagen on growth and osteogenic differentiation of bone marrow stromal cells (BMSCs). Rabbit BMSCs-populated fibrin hydrogels with or without collagen were fabricated and cultured by the free floating method for 4 weeks. The DNA content of fibrin/collagen matrix significantly increased the growth of BMSCs compared to the fibrin-only matrix at 2week. Alkaline phosphatase activity was significantly higher in the fibrin/collagen matrix (71.0 nmol of p-nitrophenol /min/disc) than the fibrin-only matrix (45.1 nmol of p-nitrophenol /min/disc). Deposition of calcium was not significantly different between two groups. Histological examination also revealed more matured organization and deposition of collagen fibers and more concentric calcium deposition in the
fibrin/collagen matrix compared to the fibrin-only matrix. These results indicated that
fibrin/collagen matrix could be more effective than fibrin alone in supporting growth and osteogenic differentiation of BMSCs. |
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