Paper Title:
Two-Dimensional Array Formation of Multi-Cellular Spheroids on Micro-Patterned Polymer Brush Surface
  Abstract

Here, a two-dimensional microarray of ten thousand (100x100) hepatocyte hetero-spheroids, underlaid with endothelial cells, was successfully constructed with a 100 µm spacing in an active area of 20x20 mm on micro-fabricated glass substrates coated with poly(ethylene glycol) (PEG) brushes (Fig. 1). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100 µm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of TBB and CBB to detect a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
449-452
DOI
10.4028/www.scientific.net/KEM.288-289.449
Citation
H. Otsuka, T. Satomi, A. Hirano, Y. Nagasaki, K. Kataoka, "Two-Dimensional Array Formation of Multi-Cellular Spheroids on Micro-Patterned Polymer Brush Surface", Key Engineering Materials, Vols. 288-289, pp. 449-452, 2005
Online since
June 2005
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Price
$32.00
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