The new-style injectable root canal filling material was prepared by hydroxyl apatite (HA), calcium hydroxide (Ca (OH) 2), iodoform (CHI3) and silicon oil. The material was injected into the isolate teeth to observe the sealing effect through marinating the isolate teeth in SBF and 1% methylblue solution. The practical effect of this material through clinical trials ten patients including fourteen teeth was also evaluated. After six months follow-up, patients have no any uncomfortable. This root canal filling material has good injectability, good biocompatibility, and is very convenient to manipulate. The relevant results suggest that it has good prospect of clinical application.