Paper Title:
Protection of Human Fibroblasts from Reactive Oxygen Species by Green Tea Polyphenolic Compounds
  Abstract

The potential protective roles played by green tea compounds (GTPCs) against reactive oxygen species-induced oxidative stress in cultured fetal human dermal fibroblasts (fHDFs) were investigated according to cell viability measurement methods, such as fluorescence double staining followed by flow cytometry (FCM), MTT assay and crystal violet uptake. Oxidative stress was induced in the fHDFs, either by adding 50 mM H2O2 or by the action of 40 U/L xanthine oxidase (XO) in the presence of xanthine (250 µM). FCM analysis was the most suitable to show that both treatments produced a significant (p < 0.05) reduction in the fHDF viability, attributed to its high sensitivity. On the microscopic observations, the cell death with necrotic morphology was appreciably induced by both treatments. These oxidative stress-induced damages were significantly (p < 0.05) prevented by pre-incubating the fHDFs with 200 µg/ml GTPC for 1 h. These results suggest that GTPC can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in cells.

  Info
Periodical
Key Engineering Materials (Volumes 288-289)
Edited by
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Pages
665-668
DOI
10.4028/www.scientific.net/KEM.288-289.665
Citation
D. W. Han, H.H. Kim, H. J. Son, H. S. Baek, K. Y. Lee, S. H. Hyon, J. C. Park, "Protection of Human Fibroblasts from Reactive Oxygen Species by Green Tea Polyphenolic Compounds", Key Engineering Materials, Vols. 288-289, pp. 665-668, 2005
Online since
June 2005
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Price
$32.00
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