Fretting is a potential degradation mechanism of structural components and equipments exposed to various environments and loading conditions. It is well known that the fatigue life under fretting condition decreases approximately 50-70% compared with that under non-fretting fatigue condition. The specific gravity of titanium alloy is 4.5 which is lighter than steel, however, its specific strength, heat and corrosion resistance are superior to steel. Ti-6Al-4V alloy is a kind of a+b phase titanium alloy, and mechanical properties are changed by alloy elements, shapes and distributions of microstructures. In this study, three different kinds of specimens are prepared under different heat treatments in order to produce different microstructures. Through various kinds of mechanical tests, the following conclusions are observed: 1) The microstructures are observed as equiaxed, bimodal and lamellar microstructures respectively. 2) The elongation percentage is superior for the equiaxed microstructure, and the hardness and tensile strength are superior for the lamellar microstructure. 3) The plain fatigue limit of lamellar structure shows higher value than that of the equiaxed and bimodal structures. 4) The fretting fatigue limit considerably decreases compared with the plain fatigue limit for all materials. 5) The fretting damage of contact surface increases with an increase of cyclic loading amplitude under the constant contact pressure.