Prevention of slack in fastening bolt has been an important researching subject since fatigue failure can be generally caused by loosening nut. According to the above reason, the super lock bolt with double screw mechanism, which is non-loosening, has been developed for preventing such kind of bolt failure. The objects of the present study are as follows: (1) To clarify the fatigue strength characteristics. (2) To investigate the initiation and propagation behaviour of fatigue crack regarding to the thread root of first pitch. In addition, the mechanism of “fatigue strength of super lock bolt” is also discussed in this paper and it is confirmed that this bolt shows excellent fatigue strength and non-loosening properties.