Crack growth behavior of S45C notched tubular specimen was studied to predict fatigue crack initiation and crack propagation under biaxial loading conditions. Stress-strain field near the hole was analyzed by ANSYS. The crack initiation lives and the crack initiation locations were predicted from strain based theories, and the analysis results were compared with the test results. Crack propagation behaviors were studied to understand the reason of crack branching and crack growth rates changing under biaxial loading conditions. Crack growth direction was also observed to find the governing factors of the fatigue damage under biaxial loading conditions.